2024 Caco-2 and ht29 cells

Caco-2 and ht29 cells

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WebNov 6, 2014 · Two reporter cell lines based on IECs, Caco-2, and HT-29 were generated, which express GFP as a reporter of NF-κB activation. The response of these novel reporter cell lines was extensively characterized by flow cytometry and validated using lactic acid bacteria (LAB) with immunomodulatory properties [ 10 , 11 ] and a natural peptide with … WebBiochem/physiol Actions. Caco-2 (Cancer coli-2) is derived from colorectal adenocarcinoma and grows as an adherent monolayer of epithelial cells. [ 6] Caco-2 cell line is a widely used intestinal epithelium model and has unique spontaneous differentiation and confluence property. It actively transports vitamins, hormones, amino acids and sugars.

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WebIn the present study, the cytotoxic response induced by D. salina biomass on the human adenocarcinoma cell lines Caco-2 and HT29 was different. Caco-2 cells were twice as … WebDec 20, 2024 · A) Ratio of Absorbance of cell culture medium (415 and 560 nm) of HT29-MTX-E12 and Caco-2 cells under static and SWMS conditions measured at 5% CO 2. B) Medium pH of apical and basolateral compartments of HT29-MTX-E12 cells grown under static and SWMS conditions at t = 1–14 days. * p < 0.05; ** p < 0.01, n = 3 C) Standard … sql date in where

Development of a serum-free co-culture of human intestinal epithelium ...

WebBriefly, Caco-2 and HT29-MTX-E12 cells were seeded in transwell inserts (12-well format) and grown for 21 days to allow differentiation of Caco-2 and mucus production by HT29-MTX-E12 cells. For the stable triple culture, the inserts were transferred onto PMA-differentiated THP-1 cells seeded in 12-well plates (WT, CASP1 -/- and NLRP3 -/- , at ... WebApr 10, 2011 · The HT29 cell line, however, can be treated with methotrexate (MTX) to differentiate the cells into mucin-secreting goblet cells [138,139]. The production of mucus by HT29-MTX cells increase adhesion of bacterial cells relative to Caco-2 or HT29 cells alone [140,141], further supporting the importance of the presence of mucus to bacterial … WebSep 21, 2024 · The cell model adopted consisted of co-cultured Caco-2 and HT29-MTX cells, while inflammatory conditions were mimicked through the incubation of epithelial cells with the conditioned medium of activated macrophages (MCM). The epithelial barrier function was monitored through trans-epithelial electrical resistance (TEER), and ROS … sql date in current month

Usefulness of Caco-2/HT29-MTX and Caco-2/HT29-MTX/Raji B …

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WebCaco-2, HT29, and SW620 cells were grown in Dulbecco’s modified Eagle medium supplemented with 10% heat-inactivated fetal calf serum, 50 mmol/L HEPES, 2 mmol/L glutamine and antibiotics in a water-saturated atmosphere of 90% air and 10% CO,. To determine cytokine WebPopular answers (1) Both of the cell lines are derived from Intestinal cancer, Caco-2 differentiates to absorptive type of cell while the HT-29 to mucus … sql date from stringWebA commercial casein hydrolysate, which contains peptides RYLGY and AYFYPEL as active molecules, has shown antihypertensive effects after acute and long-term administration. … sql date in the last 30 days

"WebPage -2 of 3-A. Preparation of the cells for transfection The day before transfection: 1. Count and measure cells for density and viability. 2. Plate 5.0 x 104 - 8.0 x 104 cells per well in 1 mL of complete growth medium (EMEM + 20% FBS). Cell density should be 50 - 80% confluent on the day of transfection. 3. Incubate cells overnight at 37°C ... " - Caco-2 and ht29 cells

Caco-2 and ht29 cells

基于十字花科素的纳米粒子与Caco-2细胞和Caco-2 / HT29-MTX共 …

WebChemical characterization has shown methyl methanethiosulfinate as major sulphurous compounds. A. roseum bulbs essential oil provides interesting antiproliferative activity against two human colonic adenocarcinoma HT29 and CACO2 cell lines in dose-dependent manner with a half-maximal inhibition (IC50) of 4.64 µg/mL and 8.22 µg/mL respectively. WebUnder these conditions, p.e. growth in glucose-free medium, cell differentiation is very similar to that observed in Caco-2 cells but in HT29 the time course of the differentiation process is longer (30 days vs. …

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WebPrior to the uptake and transport studies, Caco-2, HT-29 and HT29-MTX cell lines, and co-culture combinations thereof, were evaluated for their capacity to replicate a functional intestinal epithelium. Accordingly, mucus secretion, the morphology of the epithelium, tight-junction protein expression and the barrier integrity were evaluated. ... Web1 day ago · The penetration of FITC-dextran through the Caco-2 monolayer was also seen to be 1.5-fold higher when the cells were cultured in conditioned medium from HT29-I cells than from HT29-N cells (Fig. 6D).

WebThe most physiological relevant ratios are between 90:10 and 75:25 (Caco-2/HT-29MTX). In this range, the best compromise between model response and the presence of mucus …

WebOct 7, 2024 · The TEER values of ≈ 500 Ω * cm 2 for co-cultured and Caco-2 cells and ≈ 300 Ω * cm 2 for HT29-MTX cells are in accordance with previously published results and suggest that the cells form an intact … WebOct 6, 2024 · Cell Culture. Caco-2 (p44-48) and HT29-MTX cells (p32-40) were cultured in 75-cm 2 2 in 1.0 μm pore size cell culture inserts (PET, Falcon) in a 12 Well Tissue Culture Plate (Falcon). Cocultures were maintained for 21 days to allow Caco-2 cells to differentiate and develop enterocyte-like features. All cultures were kept at 37 °C in 5% CO 2 ...

WebThe staining study confirmed that mucus was successfully produced by HT29 cells, and it was also produced in co-cultures with Caco-2 cells model, but the Caco-2 monocultures …

WebNational Center for Biotechnology Information sql date is incompatible with intWebPrior to the uptake and transport studies, Caco-2, HT-29 and HT29-MTX cell lines, and co-culture combinations thereof, were evaluated for their capacity to replicate a functional … sql date lower thanWebApr 10, 2024 · While Caco-2/AT cells produced the highest viral titer of 251 5.1x106 PFU/ml within two days of co-culture, Vero E6 and Vero E6/AT cells took three and four 252 days to produce the highest viral titer of 1.8x105 and 5.6x104 PFU/ml, respectively (Figure 6E). 253 Together, these results indicate that Caco-2/AT cells provide an … sql date of birthWebJul 1, 2014 · In Fig. 1, Caco-2 cells were seeded in 0.33 cm 2 polycarbonate membrane at densities ranging from 5000 to 30,000 cells per well. HT29-MTX were added two days after Caco-2 seeding at a constant density of 10,000 cells per well. In co-cultures, the apparent permeability of rhodamine 123 from basolateral to apical compartments was significantly … sql date month and yearWebApr 13, 2024 · HT-29 and Caco-2-derived spheroids exhibit different spheroidization time and morphological characteristics. First, we tested whether HT-29 and Caco-2 cell lines … sql date month functionWebAlthough Caco-2 cells have been found to express a large number of enzymes and transporter proteins present in normal human intestinal epithelium, recent studies suggest that there are variations between … sql date more than 30 daysWebOct 1, 2007 · Abstract. Aim: Evaluation of adherence and invasion of Aeromonas spp. to human colon carcinoma cell lines Caco‐2 and HT29 and assessment of cytotoxic activity. Methods and Results: A number of 27 strains of Aeromonas caviae and 23 strains of Aeromonas hydrophila was analysed. All strains were capable to adhere to sub‐confluent … sql date of birth data type