WebStaining protocol: 1. Permeablize with 0.25% TritonX-100 2. Wash in PBS with 0.05% tween 20, 3 times, 5 minutes each. 3. Block with 10% donkey serum for 1 hour. ~ At this point, this section was... WebIncubate for 1 to 2 h at RT or overnight (ON) at 4°C in the dark. Wash coverslip with 1X PBS 3 times for 3 min each. Apply corresponding fluorochrome-labeled secondary antibody diluted in antibody dilution buffer and incubate for 1 h at room temperature in a moist environment in the dark. Wash coverslip with 1X PBS 3 times for 3 min each.

Efficient Delivery of Antibodies by His6-metal assembly IJN

WebCy®3 is an orange fluorophore commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. The dye has an absorption wavelength that peaks around 548-552 nm, and an … WebDec 9, 2024 · The exclusive staining of secretory granules of mucous neck cells with the lectin GSA-II as well as the antibody HIK1083 ... Cy3 was imaged using a dedicated Cy3 filter (exciter HQ 545/30, dichroic mirror Q 570 LP and emitter HQ 610/75; F41-007, Chroma Brattleboro, VT, USA; delivered by AHF analysentechnik AG, Tübingen, Germany) … breech\u0027s on

IF Staining Protocols Proteintech Group - ptglab

WebStochastic switching of Cy5 was achieved through transfer of energy from Cy3. Since then, however, a vast number of commercially available organic fluorophores were found to have photoswitching properties on their own, without relying on a dye pair (this technique is referred to as direct STORM, or dSTORM). ... list of STORM-compatible dyes: AF ... WebIncubate for 1 to 2 h at RT or overnight (ON) at 4°C in the dark. Wash coverslip with 1X PBS 3 times for 3 min each. Apply corresponding fluorochrome-labeled secondary … WebStreptavidin−Cy3 ™ from Streptomyces avidinii has been used: in double immunofluorescence staining to detect biotinylated lectin; for insulin and C-peptide … breech\\u0027s oq