Pbr9_mcherry_cre
Splet28. apr. 2024 · pBR9_mCherry_Cre (Chlamydomonas Resource Center (CRC), Saint Paul, MN, USA) by using Xho I and Bam HI restriction endonucleases, generating … SpletLOCUS pBR9_mCherry_lt.gb.xdna 5114 bp DNA circular 6/22/15 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT …
Pbr9_mcherry_cre
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Splet27. jul. 2012 · (D) Co-infection of rAAVs encoding a Cre-mCherry fusion and DO-GFP into the striatum of a wild type (WT) mouse efficiently prevents GFP expression in Cre-mCherry expressing cells. Top, a single confocal plane containing interspersed mCherry + and GFP + cells. Note that the mCherry expression is nuclear due to nuclear targeting of Cre.
http://www.zfish.cn/Article/1399.html Splet09. maj 2024 · 2. Cre-loxP重组系统诱导基因重组的方式. Cre-loxP系统存在几种诱导重组的方式，这是基于Cre重组酶与loxP位点的相互作用而实现的。. 当基因组内存在loxP位点 …
SpletHelper virus (AAV9-pEF1α-FLEX-GTB) and rabies virus [(EnvA)SAD-ΔG-mCherry] were injected into the barrel cortex of PV-Cre mice or into WT control animals. mCherry signal is visualized using the Redhot lookup table in B. (A) Rabies virus can infect PV cells and their retrogradely connected partners in the primary somatosensory cortex. SpletCodon-optimized PETase gene was substituted for the mCherry gene of pBR9_mCherry_Cre (resulting in pBR9_PETase_Cre), which is a high-strength expression vector for C. …
Splet25. sep. 2012 · Generation of the R26R-GR Allele in the Mouse. To generate a general reporter mouse, we targeted an inducible dual fluorescent protein reporter cassette (loxP-Stop-loxP-H2B-EGFP-2A-mCherry-GPI-p A; R-GR, which stands for reporter for green-red) to the R26 locus using a previously described strategy ().The H2B-EGFP encoded a histone …
SpletWe further generated transgenic zebrafish that express ubiquitous 4-hydroxytamoxifen-controlled Cre recombinase activity from a ubi:cre(ERt2) transgene, as well as ubi:loxP-EGFP-loxP-mCherry (ubi:Switch) transgenics and show their use as a constitutive fluorescent lineage tracing reagent. The ubi promoter and the transgenic lines presented … charinna\u0027s dressed chickenSpletMCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. … charinko world 2022SpletTo express monomeric untargeted mCherry, we sub-cloned the cDNA into our ble–2A expression vector, pBR9 to generate a ble–2A–mCherry fusion. The pBR9 expression vector transcriptionally fuses mCherry to the sh-ble zeocin resistance selection marker via the FMDV 2A self-cleaving sequence (Ryan et al., 1991), resulting in accumulation of ... harrow plumbingSpletMCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. char in phpSpletThank you for your interest in rAAV-hSyn-DIO-mCherry-WPRE-hGH polyA. Please provide us with your contact information and your local representative will contact you with a … char in malaySpletpBR9_mCherry_Cre(ChlamydomonasResourceCenter (CRC),SaintPaul,MN,USA)byusingXhoIandBamHI … harrow plumberSplet19. dec. 2014 · Introduction. G protein-coupled receptors (GPCRs) represent the largest family of membranous signaling molecules and are characterized structurally by a highly … harrow plumbing \\u0026 harrow homes maintenance