Splet27. jul. 2024 · We conclude that the PDGFRβ - P2A - CreER T2 line is a powerful new tool to target pericytes and will aid the field in gaining a deeper understanding of the role of … Splet01. nov. 2024 · For Cre efficiency, Pdgfrb CreERT2 mice were crossed with Rosa26-Tomato-STOP-GFP mice (B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J). The Cre recombinase activity was stimulated by intraperitoneal administration of 75 mg/kg tamoxifen (Sigma-Aldrich #10540-29-1) for 5 consecutive days. There was a 2-week …
PDGFRβ-P2A-CreERT2 mice: a genetic tool to target pericytes in ...
Splet17. nov. 2024 · Together, these experiments establish that Pdgfrb-CreERT2 transgenic mice are specific for pericytes and vSMCs in the postnatal retina, show no detectable Cre activity in ECs, and enable efficient genetic experiments in mural cells. SpletIn the PDGFRb-P2A-CreER T2-tdTomato mouse, an induction efficiency between 78.9 and 94.1% was achieved. TdTomato expression in the retina was restricted to PCs and … pet food shops adelaide
Comparison of endothelial promoter efficiency and specificity in …
Splet06. jul. 2024 · Here, we characterized calcium dynamics of cortical mural cells in anesthetized or awake Pdgfrb-CreERT2;Rosa26< LSL-GCaMP6s > mice and in acute … Splet12. avg. 2016 · Pdgfrb(BAC)-CreERT2 transgenic mice show no Cre activity in the endocardium or elsewhere in the cardiac endothelium and GFP reporter-labelled cells were abundant in the mutant AVCs and valves ... Splet12. mar. 2024 · From DM analysis, Pdgfrb + cells (AMP and VSM) showed that a limited number of cells have a transitional gene signature similar to that of SCMFs . This model was then validated by performing lineage-tracing experiments using the Pdgfrb CreERT2 line, which labels VSM and Pdgfra – fibroblasts . pet food shop near me cat