Web13 Nov 2014 · Illustration of the primers used to prepare RNA-seq libraries for high-throughput sequencing. (a) Generic oligonucleotide design, showing portions of each primer and corresponding function.(b) Sequences of the oligonucleotides used to generate Illumina-specific libraries.The RT and template-switching primers are shown at the top, and the … Web31 Jul 2024 · A strand-switching primer (SSP) present in the reaction binds to these non-templated Cs, and the reverse transcriptase then switches template from the RNA to the …
Reverse transcriptase template switching: a SMART …
WebAbstract. Here, we describe a fast, simple method for constructing full-length cDNA libraries using SMART technology. This novel procedure uses the template-switching activity of … Web6 Apr 2024 · Strand switch primer addition, with overhanging anchor sequence PCR amplification, using primers that are complementary to the anchor sequences In the case … stuart raceway iowa
Viral Sequencing, from Gunk to Graph (Two-step, strand-switching)
Web24 Dec 2024 · Strand-switching (similar to 5’ rapid amplification of cDNA ends [RACE]) only needs one primer-binding site for the synthesis of full-length cDNA, which is advantageous for sequencing methods by reducing the chances of primer-binding mismatches and bioinformatic complexity to reassemble the sequences. 57 However, not all viruses have … During first-strand synthesis when priming with an oligo dT primer to capture polyadenylated transcripts, upon reaching the 5' end of the RNA template, the terminal transferase activity of the MMLV reverse transcriptase adds a few additional nucleotides (mostly deoxycytidine) to the 3' end of the newly … See more Composition:Chimeric DNA/RNA Oligo Ordering path: Order as a Custom RNA Oligo Typical length:~30 bases Scales: 100 nmol to large … See more Emerging high-throughput technologies such as RNA-seq have enabled functional dissection of complex transcriptomes with a minimal amount of RNA input. Quantification of … See more The simple version of a TS Oligo is a DNA oligo sequence that carries 3 riboguanosines (rGrGrG) at its 3' end [1]. The complementarity … See more Described originally in 2001, a strategy frequently referred to as “SMART” (switching mechanism at the 5' end of the RNA transcript) technology (Takara Bio USA, Inc) has shown … See more WebThe transcript strand is preserved (Fig. 1a). We observe a good fraction of the reference transcripts covered by individual reads up to ~5 kb (Fig. 1b). The presence of the reverse transcription and strand-switching primer sequences in the cDNA sequences allows us to select these full-length reads from the dataset (52.2% of total reads). stuart rabner chief justice nj